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Valiant Co Ltd fastprep 24 lysing d matrix tubes
Fastprep 24 Lysing D Matrix Tubes, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 99/100, based on 8896 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysing+matrix+d+tubes/FastPrep-24+Classic/pmc13128116-231-12-20
Average 99 stars, based on 8896 article reviews
fastprep 24 lysing d matrix tubes - by Bioz Stars, 2026-09
99/100 stars

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Homogenization:

Article Title: Chemotherapy-Induced Oral Mucosal Injury Is Defined by p53 Activation, Cell Cycle Arrest and Diverse Epithelial Progenitor Dynamics
Article Snippet: Protein extracts were prepared from tissues homogenized on ice in radioimmunoprecipitation assay (RIPA) buffer (MilliporeSigma, Burlington, MA, USA; Cat# R0278) supplemented with a protease inhibitor cocktail (Protease Inhibitor Cocktail, MilliporeSigma; Cat# P8340) and phosphatase inhibitor cocktails 1 and 2 (MilliporeSigma; Cat# P2850 and P5726, respectively) at 1:100 dilution. .. Homogenization was performed using Lysing Matrix D tubes (MP Biomedicals, Irvine, CA, USA; Cat# 116913050) in a FastPrep-24TM 5G instrument (MP Biomedicals, Irvine, CA, USA; Cat# 116005500) at 6.0 m/s for 40 s, with cooling on ice between cycles. ..

Article Title: Chemotherapy-Induced Oral Mucosal Injury Is Defined by p53 Activation, Cell Cycle Arrest and Diverse Epithelial Progenitor Dynamics
Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germantown, MD, USA; Cat# 74104) according to the manufacturer’s instructions with minor modifications. .. Tissue fragments were lysed in 600 μl Buffer RLT supplemented with β-mercaptoethanol (10 μl per 1 mL; 2-mercaptoethanol; β-mercaptoethanol, Sigma-Aldrich, St. Louis, MO, USA; Cat# M6250) using Lysing Matrix D tubes (MP Biomedicals, Irvine, CA, USA; Cat# 116913050), followed by homogenization in a FastPrep-24 instrument (MP Biomedicals, Irvine, CA, USA) at 6.0 m/s for 40 s. Lysates were cleared by centrifugation (2795 x g , 1 min), mixed with an equal volume of 70% ethanol (Thermo Fisher Scientific, Waltham, MA, USA; Cat# BP28184), and applied to RNeasy spin columns. .. Columns were washed and subjected to on-column DNase digestion using the RNase-Free DNase Set (50) (Qiagen, Germantown, MD, USA; Cat# 79254) for 15 min at room temperature.

Isolation:

Article Title: A measles virus encoding a CD19/CD3 bispecific T cell engager shows enhanced preclinical anti-BCP-ALL efficacy without significant toxicity
Article Snippet: Total RNA from cell cultures, spleen, bone marrow, and meningeal cells was isolated using the Quick-RNA Miniprep Kit (Zymo Research, Irvine, CA). .. Total RNA from 30 mg tissues of IFNAR −/− CD46 Ge mouse tissues (spleen, brain, liver, and lung) was isolated using Lysing Matrix D tubes (MP Biomedicals, Santa Ana, CA), the TissueLyser LT, and the RNeasy Mini Kit (both Qiagen), according to the manufacturer’s protocols. .. RNA concentration was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific).

Article Title: Transcriptomic and proteomic insights into progressive myoclonus epilepsy, EPM1.
Article Snippet: .. Protein Isolation for LC/MS-MS For LC-MS/MS, frozen tissue samples between 20-70 mg were weighed and transferred to Lysing Matrix D tubes (MP Biomedicals) containing 600-900 μl TrizolTM Reagent (Thermo Fischer), with the volume adjusted according to tissue weight. ..

Centrifugation:

Article Title: Chemotherapy-Induced Oral Mucosal Injury Is Defined by p53 Activation, Cell Cycle Arrest and Diverse Epithelial Progenitor Dynamics
Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germantown, MD, USA; Cat# 74104) according to the manufacturer’s instructions with minor modifications. .. Tissue fragments were lysed in 600 μl Buffer RLT supplemented with β-mercaptoethanol (10 μl per 1 mL; 2-mercaptoethanol; β-mercaptoethanol, Sigma-Aldrich, St. Louis, MO, USA; Cat# M6250) using Lysing Matrix D tubes (MP Biomedicals, Irvine, CA, USA; Cat# 116913050), followed by homogenization in a FastPrep-24 instrument (MP Biomedicals, Irvine, CA, USA) at 6.0 m/s for 40 s. Lysates were cleared by centrifugation (2795 x g , 1 min), mixed with an equal volume of 70% ethanol (Thermo Fisher Scientific, Waltham, MA, USA; Cat# BP28184), and applied to RNeasy spin columns. .. Columns were washed and subjected to on-column DNase digestion using the RNase-Free DNase Set (50) (Qiagen, Germantown, MD, USA; Cat# 79254) for 15 min at room temperature.

Gene Expression:

Article Title: Development of a DUX4 -targeting antibody oligonucleotide conjugate as a therapy for FSHD
Article Snippet: .. Tissue necropsy samples for gene expression analysis were collected at the indicated time points into Lysing Matrix D tubes (MP Biomedicals, Santa Ana, CA, USA) and snap frozen on dry ice. .. Samples for RNA-induced silencing complex (RISC) loading analysis were placed into Covaris tissueTUBE cryo bags and snap frozen in liquid nitrogen.

Multiplex Assay:

Article Title: Faecalibacterium prausnitzii , depleted in the Parkinson’s disease microbiome, improves motor deficits in α-synuclein overexpressing mice
Article Snippet: .. For the multiplex bead assay, proximal large intestine segments (~2 cm) were homogenized in lysis buffer (Bio-Plex Cell Lysis Kit, Bio-Rad) using Lysing Matrix D tubes and a bead beater (MP Biomedicals). .. Total protein concentrations were quantified and normalized using the Pierce BCA Protein Assay Kit (Thermo Scientific).

Lysis:

Article Title: Faecalibacterium prausnitzii , depleted in the Parkinson’s disease microbiome, improves motor deficits in α-synuclein overexpressing mice
Article Snippet: .. For the multiplex bead assay, proximal large intestine segments (~2 cm) were homogenized in lysis buffer (Bio-Plex Cell Lysis Kit, Bio-Rad) using Lysing Matrix D tubes and a bead beater (MP Biomedicals). .. Total protein concentrations were quantified and normalized using the Pierce BCA Protein Assay Kit (Thermo Scientific).



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